59.5 Active B12
59.5.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 88.8762058 | 1 | <0.0001 | *** |
| Active.B12 | 0.0117290 | 1 | 0.9 | ns |
| GDM_new | 4.3110413 | 1 | 0.04 | * |
| Study | 0.5770705 | 1 | 0.4 | ns |
| GA_samp | 65.2025645 | 1 | <0.0001 | *** |
| Active.B12:GDM_new | 2.6142387 | 1 | 0.1 | ns |
| GDM_new:Study | 4.0486148 | 1 | 0.04 | * |
| Active.B12:Study | 0.0000391 | 1 | 1.0 | ns |
| Active.B12:GDM_new:Study | 2.7234579 | 1 | 0.10 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 12.1235530 | 1 | 0.0005 | *** |
| Active.B12 | 0.0056038 | 1 | 0.9 | ns |
| GDM_new | 9.9531213 | 1 | 0.002 | ** |
| Study | 2.2266179 | 1 | 0.1 | ns |
| GA_samp | 1064.5741775 | 1 | <0.0001 | *** |
| Active.B12:GDM_new | 6.5685275 | 1 | 0.01 | * |
| GDM_new:Study | 6.1820118 | 1 | 0.01 | * |
| Active.B12:Study | 0.0261088 | 1 | 0.9 | ns |
| Active.B12:GDM_new:Study | 4.0649939 | 1 | 0.04 | * |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 185.124725 | 1 | <0.0001 | *** |
| Active.B12 | 1.478688 | 1 | 0.2 | ns |
| GDM_new | 3.592857 | 1 | 0.06 | ns |
| Study | 1.010315 | 1 | 0.3 | ns |
| GA_samp | 494.549744 | 1 | <0.0001 | *** |
| Active.B12:GDM_new | 1.696353 | 1 | 0.2 | ns |
| GDM_new:Study | 2.413833 | 1 | 0.1 | ns |
| Active.B12:Study | 2.065497 | 1 | 0.2 | ns |
| Active.B12:GDM_new:Study | 1.019242 | 1 | 0.3 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Active.B12 | 1 | Inf | 2.222 | 2.222 | 0.1 | ns |
| 5 | GDM_new | 1 | Inf | 12.975 | 12.975 | 0.0003 | *** |
| 7 | Study | 1 | Inf | 4.880 | 4.880 | 0.03 | * |
| 8 | GA_samp | 1 | Inf | 0.005 | 0.005 | 0.9 | ns |
| 2 | Active.B12:GDM_new | 1 | Inf | 1.540 | 1.540 | 0.2 | ns |
| 4 | Active.B12:Study | 1 | Inf | 0.565 | 0.565 | 0.5 | ns |
| 6 | GDM_new:Study | 1 | Inf | 1.674 | 1.674 | 0.2 | ns |
| 3 | Active.B12:GDM_new:Study | 1 | Inf | 0.457 | 0.457 | 0.5 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Active.B12 | 1 | Inf | 2.321 | 2.321 | 0.1 | ns |
| 5 | GDM_new | 1 | Inf | 11.866 | 11.866 | 0.0006 | *** |
| 7 | Study | 1 | Inf | 6.577 | 6.577 | 0.01 | * |
| 8 | GA_samp | 1 | Inf | 0.145 | 0.145 | 0.7 | ns |
| 2 | Active.B12:GDM_new | 1 | Inf | 1.586 | 1.586 | 0.2 | ns |
| 4 | Active.B12:Study | 1 | Inf | 0.697 | 0.697 | 0.4 | ns |
| 6 | GDM_new:Study | 1 | Inf | 1.694 | 1.694 | 0.2 | ns |
| 3 | Active.B12:GDM_new:Study | 1 | Inf | 0.521 | 0.521 | 0.5 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Active.B12 | 1 | Inf | 1.286 | 1.286 | 0.3 | ns |
| 5 | GDM_new | 1 | Inf | 3.887 | 3.887 | 0.05 | ns |
| 7 | Study | 1 | Inf | 8.371 | 8.371 | 0.004 | ** |
| 8 | GA_samp | 1 | Inf | 1.478 | 1.478 | 0.2 | ns |
| 2 | Active.B12:GDM_new | 1 | Inf | 3.835 | 3.835 | 0.05 | ns |
| 4 | Active.B12:Study | 1 | Inf | 1.680 | 1.680 | 0.2 | ns |
| 6 | GDM_new:Study | 1 | Inf | 1.989 | 1.989 | 0.2 | ns |
| 3 | Active.B12:GDM_new:Study | 1 | Inf | 1.380 | 1.380 | 0.2 | ns |
59.5.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.75 (2.17, 3.33) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.51 |
| GDM_newGDM | -0.30 (-0.76, 0.16) | 0.20 |
| StudySTOP | -0.04 (-0.34, 0.27) | 0.82 |
| GA_samp | 0.14 (0.10, 0.17) | <0.0001 |
| Active.B12:GDM_newGDM | 0.00 (-0.00, 0.01) | 0.71 |
| GDM_newGDM:StudySTOP | 0.22 (-0.10, 0.54) | 0.18 |
| Active.B12:StudySTOP | -0.00 (-0.01, 0.00) | 0.50 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 0.54 (0.24, 0.85) | 0.0005 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.94 |
| GDM_newGDM | -0.64 (-1.03, -0.24) | 0.002 |
| StudySTOP | 0.13 (-0.04, 0.31) | 0.14 |
| GA_samp | 0.30 (0.28, 0.32) | <0.0001 |
| Active.B12:GDM_newGDM | 0.01 (0.00, 0.01) | 0.01 |
| GDM_newGDM:StudySTOP | 0.58 (0.12, 1.04) | 0.01 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.87 |
| Active.B12:GDM_newGDM:StudySTOP | -0.01 (-0.01, -0.00) | 0.04 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | -2.78 (-3.17, -2.38) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.11 |
| GDM_newGDM | -0.30 (-0.63, 0.02) | 0.07 |
| StudySTOP | 0.15 (-0.06, 0.37) | 0.16 |
| GA_samp | 0.27 (0.24, 0.29) | <0.0001 |
| Active.B12:GDM_newGDM | 0.00 (-0.00, 0.01) | 0.40 |
| GDM_newGDM:StudySTOP | 0.19 (-0.03, 0.42) | 0.09 |
| Active.B12:StudySTOP | -0.00 (-0.01, -0.00) | 0.05 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.76 (2.02, 3.50) | <0.0001 |
| Active.B12 | -0.00 (-0.01, 0.00) | 0.60 |
| GDM_newGDM | 1.29 (0.68, 1.90) | <0.0001 |
| StudySTOP | -0.39 (-0.81, 0.03) | 0.07 |
| GA_samp | -0.00 (-0.04, 0.04) | 0.98 |
| Active.B12:GDM_newGDM | -0.00 (-0.01, 0.00) | 0.29 |
| GDM_newGDM:StudySTOP | -0.42 (-0.86, 0.01) | 0.06 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.01) | 0.67 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.47 (0.66, 2.27) | 0.0003 |
| Active.B12 | -0.00 (-0.01, 0.00) | 0.55 |
| GDM_newGDM | 1.39 (0.69, 2.08) | <0.0001 |
| StudySTOP | -0.58 (-1.04, -0.12) | 0.01 |
| GA_samp | -0.01 (-0.05, 0.04) | 0.74 |
| Active.B12:GDM_newGDM | -0.00 (-0.01, 0.00) | 0.28 |
| GDM_newGDM:StudySTOP | -0.46 (-0.95, 0.03) | 0.07 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.01) | 0.61 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.77 (1.62, 1.91) | <0.0001 |
| Active.B12 | 0.00 (-0.00, 0.00) | 0.89 |
| GDM_newGDM | 0.29 (-0.01, 0.59) | 0.06 |
| StudySTOP | -0.20 (-0.29, -0.11) | <0.0001 |
| GA_samp | -0.00 (-0.01, 0.00) | 0.35 |
| Active.B12:GDM_newGDM | -0.00 (-0.00, -0.00) | 0.04 |
| GDM_newGDM:StudySTOP | -0.13 (-0.31, 0.05) | 0.15 |
| Active.B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.32 |
59.5.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 102.86520 | 9.521582 | 137.37705 | 85.65996 | 123.5262 |
| STOP | Uncomplicated | 90.21700 | 7.582765 | 95.51545 | 76.35309 | 106.5983 |
| SCOPE | GDM | 81.00543 | 13.173215 | 541.77161 | 58.85463 | 111.4930 |
| STOP | GDM | 88.41300 | 9.441899 | 216.02677 | 71.63109 | 109.1266 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 87.99884 | 3.073679 | 301.7892 | 82.15347 | 94.26012 |
| STOP | Uncomplicated | 101.77698 | 2.986908 | 161.3797 | 96.04619 | 107.84972 |
| SCOPE | GDM | 71.62685 | 5.600898 | 491.5282 | 61.42588 | 83.52188 |
| STOP | GDM | 99.79654 | 4.557162 | 397.7761 | 91.22779 | 109.17014 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 2.330578 | 0.0998808 | 363.9985 | 2.142211 | 2.535509 |
| STOP | Uncomplicated | 2.238491 | 0.0770037 | 208.2326 | 2.091718 | 2.395564 |
| SCOPE | GDM | 1.908238 | 0.1917335 | 555.2210 | 1.566461 | 2.324584 |
| STOP | GDM | 2.227019 | 0.1242820 | 383.5627 | 1.995588 | 2.485288 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 14.42651 | 1.1311375 | Inf | 12.371472 | 16.82292 |
| STOP | Uncomplicated | 10.63751 | 0.6140156 | Inf | 9.499639 | 11.91167 |
| SCOPE | GDM | 40.14113 | 7.5536756 | Inf | 27.759541 | 58.04529 |
| STOP | GDM | 19.37977 | 2.1358081 | Inf | 15.614899 | 24.05237 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 3.520364 | 0.2997609 | Inf | 2.979252 | 4.159756 |
| STOP | Uncomplicated | 2.208426 | 0.1354540 | Inf | 1.958278 | 2.490528 |
| SCOPE | GDM | 10.487870 | 2.2799730 | Inf | 6.849256 | 16.059471 |
| STOP | GDM | 4.153545 | 0.4913196 | Inf | 3.294056 | 5.237294 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 5.527781 | 0.0917408 | Inf | 5.350866 | 5.710546 |
| STOP | Uncomplicated | 4.761846 | 0.0623612 | Inf | 4.641176 | 4.885654 |
| SCOPE | GDM | 6.409661 | 0.5594051 | Inf | 5.401895 | 7.605433 |
| STOP | GDM | 4.843211 | 0.1023149 | Inf | 4.646773 | 5.047954 |
59.5.4 Comparisons
59.5.4.1 Within Groups
Effect for every 10 unit increase in Active B12 concentration (pM).| Hormone | Contrast | Study | GDM_new | Ratio of Geometric means (95% CI) | P-value |
|---|---|---|---|---|---|
| Prolactin | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 1.011 (0.978, 1.046) | 0.5 |
| Prolactin | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 0.997 (0.970, 1.025) | 0.8 |
| Prolactin | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.021 (0.966, 1.078) | 0.5 |
| Prolactin | Active.B1271 / Active.B1261 | STOP | GDM | 1.006 (0.962, 1.053) | 0.8 |
| HPL | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 1.001 (0.982, 1.020) | 0.9 |
| HPL | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 1.003 (0.987, 1.018) | 0.7 |
| HPL | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.068 (1.020, 1.118) | 0.005 |
| HPL | Active.B1271 / Active.B1261 | STOP | GDM | 1.008 (0.981, 1.036) | 0.6 |
| GH2 | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 1.019 (0.996, 1.043) | 0.1 |
| GH2 | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 0.990 (0.970, 1.009) | 0.3 |
| GH2 | Active.B1271 / Active.B1261 | SCOPE | GDM | 1.035 (0.996, 1.076) | 0.08 |
| GH2 | Active.B1271 / Active.B1261 | STOP | GDM | 1.005 (0.973, 1.038) | 0.8 |
| Insulin | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 0.987 (0.942, 1.035) | 0.6 |
| Insulin | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 1.000 (0.961, 1.040) | 1.0 |
| Insulin | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.948 (0.875, 1.027) | 0.2 |
| Insulin | Active.B1271 / Active.B1261 | STOP | GDM | 0.960 (0.897, 1.028) | 0.2 |
| HOMA.IR | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 0.984 (0.935, 1.036) | 0.5 |
| HOMA.IR | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 1.001 (0.959, 1.044) | 1.0 |
| HOMA.IR | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.941 (0.861, 1.028) | 0.2 |
| HOMA.IR | Active.B1271 / Active.B1261 | STOP | GDM | 0.957 (0.888, 1.031) | 0.2 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | SCOPE | Uncomplicated | 1.001 (0.991, 1.011) | 0.9 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | STOP | Uncomplicated | 1.008 (0.998, 1.019) | 0.1 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | SCOPE | GDM | 0.980 (0.955, 1.004) | 0.1 |
| GLUC3_mmol.L | Active.B1271 / Active.B1261 | STOP | GDM | 0.987 (0.970, 1.004) | 0.1 |
59.5.4.2 Between Groups
Below are comparisons of the differences in the change in Active B12 concentration (pM) between groups. I.e. whether the change in Active B12 concentration (pM) are different between groups.
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9714900 | 0.0413634 | 702.3499 | 0.8935810 | 1.056192 | 0.497 | ns |
| STOP/SCOPE (Uncomplicated) | 0.9856419 | 0.0209830 | 702.3499 | 0.9452941 | 1.027712 | 0.497 | ns |
| STOP/SCOPE (GDM) | 0.9856419 | 0.0209830 | 702.3499 | 0.9452941 | 1.027712 | 0.497 | ns |
| GDM/Uncomplicated (SCOPE) | 1.0094327 | 0.0257837 | 688.1093 | 0.9600570 | 1.061348 | 0.713 | ns |
| GDM/Uncomplicated (STOP) | 1.0094327 | 0.0257837 | 688.1093 | 0.9600570 | 1.061348 | 0.713 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9455281 | 0.0286475 | 699.3782 | 0.8909229 | 1.0034800 | 0.065 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0019949 | 0.0123586 | 714.0996 | 0.9780228 | 1.0265546 | 0.872 | ns |
| STOP/SCOPE (GDM) | 0.9436455 | 0.0258363 | 717.4804 | 0.8942609 | 0.9957574 | 0.034 | * |
| GDM/Uncomplicated (SCOPE) | 1.0673053 | 0.0271258 | 725.2300 | 1.0153576 | 1.1219107 | 0.011 | * |
| GDM/Uncomplicated (STOP) | 1.0051526 | 0.0161172 | 692.7746 | 0.9740011 | 1.0373005 | 0.749 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9428995 | 0.0282910 | 723.6672 | 0.8889615 | 1.000110 | 0.050 | ns |
| STOP/SCOPE (Uncomplicated) | 0.9710301 | 0.0145675 | 723.6672 | 0.9428475 | 1.000055 | 0.050 | ns |
| STOP/SCOPE (GDM) | 0.9710301 | 0.0145675 | 723.6672 | 0.9428475 | 1.000055 | 0.050 | ns |
| GDM/Uncomplicated (SCOPE) | 1.0156009 | 0.0185906 | 725.0212 | 0.9797511 | 1.052763 | 0.398 | ns |
| GDM/Uncomplicated (STOP) | 1.0156009 | 0.0185906 | 725.0212 | 0.9797511 | 1.052763 | 0.398 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0256798 | 0.0615096 | Inf | 0.9119385 | 1.153607 | 0.672 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0127585 | 0.0303674 | Inf | 0.9549547 | 1.074061 | 0.672 | ns |
| STOP/SCOPE (GDM) | 1.0127585 | 0.0303674 | Inf | 0.9549547 | 1.074061 | 0.672 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9603312 | 0.0363751 | Inf | 0.8916195 | 1.034338 | 0.285 | ns |
| GDM/Uncomplicated (STOP) | 0.9603312 | 0.0363751 | Inf | 0.8916195 | 1.034338 | 0.285 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0339325 | 0.0674890 | Inf | 0.9097683 | 1.175042 | 0.609 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0168247 | 0.0331861 | Inf | 0.9538178 | 1.083994 | 0.609 | ns |
| STOP/SCOPE (GDM) | 1.0168247 | 0.0331861 | Inf | 0.9538178 | 1.083994 | 0.609 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9558794 | 0.0399053 | Inf | 0.8807808 | 1.037381 | 0.280 | ns |
| GDM/Uncomplicated (STOP) | 0.9558794 | 0.0399053 | Inf | 0.8807808 | 1.037381 | 0.280 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.015044 | 0.0152556 | Inf | 0.9855796 | 1.0453889 | 0.320 | ns |
| STOP/SCOPE (Uncomplicated) | 1.007494 | 0.0075710 | Inf | 0.9927636 | 1.0224426 | 0.320 | ns |
| STOP/SCOPE (GDM) | 1.007494 | 0.0075710 | Inf | 0.9927636 | 1.0224426 | 0.320 | ns |
| GDM/Uncomplicated (SCOPE) | 0.978799 | 0.0104166 | Inf | 0.9585944 | 0.9994295 | 0.044 | * |
| GDM/Uncomplicated (STOP) | 0.978799 | 0.0104166 | Inf | 0.9585944 | 0.9994295 | 0.044 | * |