59.5 Active B12

59.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 88.8762058 1 <0.0001 ***
Active.B12 0.0117290 1 0.9 ns
GDM_new 4.3110413 1 0.04 *
Study 0.5770705 1 0.4 ns
GA_samp 65.2025645 1 <0.0001 ***
Active.B12:GDM_new 2.6142387 1 0.1 ns
GDM_new:Study 4.0486148 1 0.04 *
Active.B12:Study 0.0000391 1 1.0 ns
Active.B12:GDM_new:Study 2.7234579 1 0.10 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 12.1235530 1 0.0005 ***
Active.B12 0.0056038 1 0.9 ns
GDM_new 9.9531213 1 0.002 **
Study 2.2266179 1 0.1 ns
GA_samp 1064.5741775 1 <0.0001 ***
Active.B12:GDM_new 6.5685275 1 0.01 *
GDM_new:Study 6.1820118 1 0.01 *
Active.B12:Study 0.0261088 1 0.9 ns
Active.B12:GDM_new:Study 4.0649939 1 0.04 *
GH2
Chisq Df Pr(>Chisq)
(Intercept) 185.124725 1 <0.0001 ***
Active.B12 1.478688 1 0.2 ns
GDM_new 3.592857 1 0.06 ns
Study 1.010315 1 0.3 ns
GA_samp 494.549744 1 <0.0001 ***
Active.B12:GDM_new 1.696353 1 0.2 ns
GDM_new:Study 2.413833 1 0.1 ns
Active.B12:Study 2.065497 1 0.2 ns
Active.B12:GDM_new:Study 1.019242 1 0.3 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 2.222 2.222 0.1 ns
5 GDM_new 1 Inf 12.975 12.975 0.0003 ***
7 Study 1 Inf 4.880 4.880 0.03 *
8 GA_samp 1 Inf 0.005 0.005 0.9 ns
2 Active.B12:GDM_new 1 Inf 1.540 1.540 0.2 ns
4 Active.B12:Study 1 Inf 0.565 0.565 0.5 ns
6 GDM_new:Study 1 Inf 1.674 1.674 0.2 ns
3 Active.B12:GDM_new:Study 1 Inf 0.457 0.457 0.5 ns
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 2.321 2.321 0.1 ns
5 GDM_new 1 Inf 11.866 11.866 0.0006 ***
7 Study 1 Inf 6.577 6.577 0.01 *
8 GA_samp 1 Inf 0.145 0.145 0.7 ns
2 Active.B12:GDM_new 1 Inf 1.586 1.586 0.2 ns
4 Active.B12:Study 1 Inf 0.697 0.697 0.4 ns
6 GDM_new:Study 1 Inf 1.694 1.694 0.2 ns
3 Active.B12:GDM_new:Study 1 Inf 0.521 0.521 0.5 ns
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 1.286 1.286 0.3 ns
5 GDM_new 1 Inf 3.887 3.887 0.05 ns
7 Study 1 Inf 8.371 8.371 0.004 **
8 GA_samp 1 Inf 1.478 1.478 0.2 ns
2 Active.B12:GDM_new 1 Inf 3.835 3.835 0.05 ns
4 Active.B12:Study 1 Inf 1.680 1.680 0.2 ns
6 GDM_new:Study 1 Inf 1.989 1.989 0.2 ns
3 Active.B12:GDM_new:Study 1 Inf 1.380 1.380 0.2 ns

59.5.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.75 (2.17, 3.33) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.51
GDM_newGDM -0.30 (-0.76, 0.16) 0.20
StudySTOP -0.04 (-0.34, 0.27) 0.82
GA_samp 0.14 (0.10, 0.17) <0.0001
Active.B12:GDM_newGDM 0.00 (-0.00, 0.01) 0.71
GDM_newGDM:StudySTOP 0.22 (-0.10, 0.54) 0.18
Active.B12:StudySTOP -0.00 (-0.01, 0.00) 0.50
HPL
Var Effect P
(Intercept) 0.54 (0.24, 0.85) 0.0005
Active.B12 0.00 (-0.00, 0.00) 0.94
GDM_newGDM -0.64 (-1.03, -0.24) 0.002
StudySTOP 0.13 (-0.04, 0.31) 0.14
GA_samp 0.30 (0.28, 0.32) <0.0001
Active.B12:GDM_newGDM 0.01 (0.00, 0.01) 0.01
GDM_newGDM:StudySTOP 0.58 (0.12, 1.04) 0.01
Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.87
Active.B12:GDM_newGDM:StudySTOP -0.01 (-0.01, -0.00) 0.04
GH2
Var Effect P
(Intercept) -2.78 (-3.17, -2.38) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.11
GDM_newGDM -0.30 (-0.63, 0.02) 0.07
StudySTOP 0.15 (-0.06, 0.37) 0.16
GA_samp 0.27 (0.24, 0.29) <0.0001
Active.B12:GDM_newGDM 0.00 (-0.00, 0.01) 0.40
GDM_newGDM:StudySTOP 0.19 (-0.03, 0.42) 0.09
Active.B12:StudySTOP -0.00 (-0.01, -0.00) 0.05
Insulin
Var Effect P
(Intercept) 2.76 (2.02, 3.50) <0.0001
Active.B12 -0.00 (-0.01, 0.00) 0.60
GDM_newGDM 1.29 (0.68, 1.90) <0.0001
StudySTOP -0.39 (-0.81, 0.03) 0.07
GA_samp -0.00 (-0.04, 0.04) 0.98
Active.B12:GDM_newGDM -0.00 (-0.01, 0.00) 0.29
GDM_newGDM:StudySTOP -0.42 (-0.86, 0.01) 0.06
Active.B12:StudySTOP 0.00 (-0.00, 0.01) 0.67
HOMA.IR
Var Effect P
(Intercept) 1.47 (0.66, 2.27) 0.0003
Active.B12 -0.00 (-0.01, 0.00) 0.55
GDM_newGDM 1.39 (0.69, 2.08) <0.0001
StudySTOP -0.58 (-1.04, -0.12) 0.01
GA_samp -0.01 (-0.05, 0.04) 0.74
Active.B12:GDM_newGDM -0.00 (-0.01, 0.00) 0.28
GDM_newGDM:StudySTOP -0.46 (-0.95, 0.03) 0.07
Active.B12:StudySTOP 0.00 (-0.00, 0.01) 0.61
GLUC3_mmol.L
Var Effect P
(Intercept) 1.77 (1.62, 1.91) <0.0001
Active.B12 0.00 (-0.00, 0.00) 0.89
GDM_newGDM 0.29 (-0.01, 0.59) 0.06
StudySTOP -0.20 (-0.29, -0.11) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.35
Active.B12:GDM_newGDM -0.00 (-0.00, -0.00) 0.04
GDM_newGDM:StudySTOP -0.13 (-0.31, 0.05) 0.15
Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.32

59.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.

Prolactin
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 102.86520 9.521582 137.37705 85.65996 123.5262
STOP Uncomplicated 90.21700 7.582765 95.51545 76.35309 106.5983
SCOPE GDM 81.00543 13.173215 541.77161 58.85463 111.4930
STOP GDM 88.41300 9.441899 216.02677 71.63109 109.1266
HPL
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 87.99884 3.073679 301.7892 82.15347 94.26012
STOP Uncomplicated 101.77698 2.986908 161.3797 96.04619 107.84972
SCOPE GDM 71.62685 5.600898 491.5282 61.42588 83.52188
STOP GDM 99.79654 4.557162 397.7761 91.22779 109.17014
GH2
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 2.330578 0.0998808 363.9985 2.142211 2.535509
STOP Uncomplicated 2.238491 0.0770037 208.2326 2.091718 2.395564
SCOPE GDM 1.908238 0.1917335 555.2210 1.566461 2.324584
STOP GDM 2.227019 0.1242820 383.5627 1.995588 2.485288
Insulin
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 14.42651 1.1311375 Inf 12.371472 16.82292
STOP Uncomplicated 10.63751 0.6140156 Inf 9.499639 11.91167
SCOPE GDM 40.14113 7.5536756 Inf 27.759541 58.04529
STOP GDM 19.37977 2.1358081 Inf 15.614899 24.05237
HOMA.IR
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 3.520364 0.2997609 Inf 2.979252 4.159756
STOP Uncomplicated 2.208426 0.1354540 Inf 1.958278 2.490528
SCOPE GDM 10.487870 2.2799730 Inf 6.849256 16.059471
STOP GDM 4.153545 0.4913196 Inf 3.294056 5.237294
GLUC3_mmol.L
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 5.527781 0.0917408 Inf 5.350866 5.710546
STOP Uncomplicated 4.761846 0.0623612 Inf 4.641176 4.885654
SCOPE GDM 6.409661 0.5594051 Inf 5.401895 7.605433
STOP GDM 4.843211 0.1023149 Inf 4.646773 5.047954

59.5.4 Comparisons

59.5.4.1 Within Groups

Effect for every 10 unit increase in Active B12 concentration (pM).
Hormone Contrast Study GDM_new Ratio of Geometric means (95% CI) P-value
Prolactin Active.B1271 / Active.B1261 SCOPE Uncomplicated 1.011 (0.978, 1.046) 0.5
Prolactin Active.B1271 / Active.B1261 STOP Uncomplicated 0.997 (0.970, 1.025) 0.8
Prolactin Active.B1271 / Active.B1261 SCOPE GDM 1.021 (0.966, 1.078) 0.5
Prolactin Active.B1271 / Active.B1261 STOP GDM 1.006 (0.962, 1.053) 0.8
HPL Active.B1271 / Active.B1261 SCOPE Uncomplicated 1.001 (0.982, 1.020) 0.9
HPL Active.B1271 / Active.B1261 STOP Uncomplicated 1.003 (0.987, 1.018) 0.7
HPL Active.B1271 / Active.B1261 SCOPE GDM 1.068 (1.020, 1.118) 0.005
HPL Active.B1271 / Active.B1261 STOP GDM 1.008 (0.981, 1.036) 0.6
GH2 Active.B1271 / Active.B1261 SCOPE Uncomplicated 1.019 (0.996, 1.043) 0.1
GH2 Active.B1271 / Active.B1261 STOP Uncomplicated 0.990 (0.970, 1.009) 0.3
GH2 Active.B1271 / Active.B1261 SCOPE GDM 1.035 (0.996, 1.076) 0.08
GH2 Active.B1271 / Active.B1261 STOP GDM 1.005 (0.973, 1.038) 0.8
Insulin Active.B1271 / Active.B1261 SCOPE Uncomplicated 0.987 (0.942, 1.035) 0.6
Insulin Active.B1271 / Active.B1261 STOP Uncomplicated 1.000 (0.961, 1.040) 1.0
Insulin Active.B1271 / Active.B1261 SCOPE GDM 0.948 (0.875, 1.027) 0.2
Insulin Active.B1271 / Active.B1261 STOP GDM 0.960 (0.897, 1.028) 0.2
HOMA.IR Active.B1271 / Active.B1261 SCOPE Uncomplicated 0.984 (0.935, 1.036) 0.5
HOMA.IR Active.B1271 / Active.B1261 STOP Uncomplicated 1.001 (0.959, 1.044) 1.0
HOMA.IR Active.B1271 / Active.B1261 SCOPE GDM 0.941 (0.861, 1.028) 0.2
HOMA.IR Active.B1271 / Active.B1261 STOP GDM 0.957 (0.888, 1.031) 0.2
GLUC3_mmol.L Active.B1271 / Active.B1261 SCOPE Uncomplicated 1.001 (0.991, 1.011) 0.9
GLUC3_mmol.L Active.B1271 / Active.B1261 STOP Uncomplicated 1.008 (0.998, 1.019) 0.1
GLUC3_mmol.L Active.B1271 / Active.B1261 SCOPE GDM 0.980 (0.955, 1.004) 0.1
GLUC3_mmol.L Active.B1271 / Active.B1261 STOP GDM 0.987 (0.970, 1.004) 0.1

59.5.4.2 Between Groups

Below are comparisons of the differences in the change in Active B12 concentration (pM) between groups. I.e. whether the change in Active B12 concentration (pM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9714900 0.0413634 702.3499 0.8935810 1.056192 0.497 ns
STOP/SCOPE (Uncomplicated) 0.9856419 0.0209830 702.3499 0.9452941 1.027712 0.497 ns
STOP/SCOPE (GDM) 0.9856419 0.0209830 702.3499 0.9452941 1.027712 0.497 ns
GDM/Uncomplicated (SCOPE) 1.0094327 0.0257837 688.1093 0.9600570 1.061348 0.713 ns
GDM/Uncomplicated (STOP) 1.0094327 0.0257837 688.1093 0.9600570 1.061348 0.713 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9455281 0.0286475 699.3782 0.8909229 1.0034800 0.065 ns
STOP/SCOPE (Uncomplicated) 1.0019949 0.0123586 714.0996 0.9780228 1.0265546 0.872 ns
STOP/SCOPE (GDM) 0.9436455 0.0258363 717.4804 0.8942609 0.9957574 0.034 *
GDM/Uncomplicated (SCOPE) 1.0673053 0.0271258 725.2300 1.0153576 1.1219107 0.011 *
GDM/Uncomplicated (STOP) 1.0051526 0.0161172 692.7746 0.9740011 1.0373005 0.749 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9428995 0.0282910 723.6672 0.8889615 1.000110 0.050 ns
STOP/SCOPE (Uncomplicated) 0.9710301 0.0145675 723.6672 0.9428475 1.000055 0.050 ns
STOP/SCOPE (GDM) 0.9710301 0.0145675 723.6672 0.9428475 1.000055 0.050 ns
GDM/Uncomplicated (SCOPE) 1.0156009 0.0185906 725.0212 0.9797511 1.052763 0.398 ns
GDM/Uncomplicated (STOP) 1.0156009 0.0185906 725.0212 0.9797511 1.052763 0.398 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0256798 0.0615096 Inf 0.9119385 1.153607 0.672 ns
STOP/SCOPE (Uncomplicated) 1.0127585 0.0303674 Inf 0.9549547 1.074061 0.672 ns
STOP/SCOPE (GDM) 1.0127585 0.0303674 Inf 0.9549547 1.074061 0.672 ns
GDM/Uncomplicated (SCOPE) 0.9603312 0.0363751 Inf 0.8916195 1.034338 0.285 ns
GDM/Uncomplicated (STOP) 0.9603312 0.0363751 Inf 0.8916195 1.034338 0.285 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0339325 0.0674890 Inf 0.9097683 1.175042 0.609 ns
STOP/SCOPE (Uncomplicated) 1.0168247 0.0331861 Inf 0.9538178 1.083994 0.609 ns
STOP/SCOPE (GDM) 1.0168247 0.0331861 Inf 0.9538178 1.083994 0.609 ns
GDM/Uncomplicated (SCOPE) 0.9558794 0.0399053 Inf 0.8807808 1.037381 0.280 ns
GDM/Uncomplicated (STOP) 0.9558794 0.0399053 Inf 0.8807808 1.037381 0.280 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.015044 0.0152556 Inf 0.9855796 1.0453889 0.320 ns
STOP/SCOPE (Uncomplicated) 1.007494 0.0075710 Inf 0.9927636 1.0224426 0.320 ns
STOP/SCOPE (GDM) 1.007494 0.0075710 Inf 0.9927636 1.0224426 0.320 ns
GDM/Uncomplicated (SCOPE) 0.978799 0.0104166 Inf 0.9585944 0.9994295 0.044 *
GDM/Uncomplicated (STOP) 0.978799 0.0104166 Inf 0.9585944 0.9994295 0.044 *

59.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

59.5.6 Plots